Package: MBmca 1.0.1

Stefan Roediger

MBmca: Nucleic Acid Melting Curve Analysis

Lightweight utilities for nucleic acid melting curve analysis are important in life sciences and diagnostics. This software can be used for the analysis and presentation of melting curve data from microbead-based assays (surface melting curve analysis) and reactions in solution (e.g., quantitative PCR (qPCR), real-time isothermal Amplification). Further information are described in detail in two publications in The R Journal [ <https://journal.r-project.org/archive/2013-2/roediger-bohm-schimke.pdf>; <https://journal.r-project.org/archive/2015-1/RJ-2015-1.pdf>].

Authors:Stefan Roediger [cre, aut], Michal Burdukiewicz [aut]

MBmca_1.0.1.tar.gz
MBmca_1.0.1.zip(r-4.7)MBmca_1.0.1.zip(r-4.6)MBmca_1.0.1.zip(r-4.5)
MBmca_1.0.1.tgz(r-4.6-any)MBmca_1.0.1.tgz(r-4.5-any)
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MBmca_1.0.1.tgz(r-4.6-emscripten)
manual.pdf |manual.html✨
DESCRIPTION
card.svg |card.png
MBmca/json (API)

# Install 'MBmca' in R:

install.packages('MBmca', repos = c('https://pcruniversum.r-universe.dev', 'https://cloud.r-project.org'))

Bug tracker:https://github.com/pcruniversum/mbmca/issues

Datasets:

DMP - Bimodal melting curve experiment on the surface of microbeads.
DualHyb - Surface melting curve data from direct hybridization experiment of short oligonucleotide probes with bimodal melting curve pattern.
MultiMelt - Surface melting curve data from direct hybridization experiment of short oligonucleotides.

On CRAN:

5.01 score 4 stars 1 packages 17 scripts 192 downloads 5 exports 48 dependencies

Last updated from:def2bb5807. Checks:7 NOTE, 2 OK. Indexed: yes.

Target	Result	Time
linux-devel-x86_64	NOTE	161
source / vignettes	OK	170
linux-release-x86_64	NOTE	157
macos-release-arm64	NOTE	94
macos-oldrel-arm64	NOTE	116
windows-devel	NOTE	124
windows-release	NOTE	108
windows-oldrel	NOTE	101
wasm-release	OK	116

Exports:diffQ diffQ2 mcaPeaks mcaSmoother MFIerror

Dependencies:base64enc bslib cachem chipPCR cli commonmark DEoptimR digest fastmap fontawesome fs glue htmltools httpuv jquerylib jsonlite later lattice lifecycle lmtest magrittr MASS Matrix MatrixModels memoise mime otel outliers promises ptw quantreg R6 rappdirs Rcpp RcppArmadillo RcppDE Rfit rlang robustbase sass shiny signal sourcetools SparseM survival withr xtable zoo

Microbead Surface Nucleic Acid Melting Curve Analysis

References

Rendered fromMBmca.Rmdusingknitr::rmarkdown

Last update: 2021-03-07
Started: 2021-02-27

Citation

Development and contributors

Readme and manuals

Help Manual

Help page	Topics
Microbead Surface Nucleic Acid Melting Curve Analysis	MBmca-package MBmca
Calculation of the melting temperature (Tm) from the first derivative	diffQ
Calculation of the melting temperatures (Tm, Tm1D2 and Tm2D2) from the first and the second derivative	diffQ2
Bimodal melting curve experiment on the surface of microbeads.	DMP
Surface melting curve data from direct hybridization experiment of short oligonucleotide probes with bimodal melting curve pattern.	DualHyb
Function to estimate the approximate local minima and maxima of melting curve data.	mcaPeaks
Function to pre-process melting curve data.	mcaSmoother
Multiple comparison of the temperature dependent variance of the refMFI	MFIerror
Surface melting curve data from direct hybridization experiment of short oligonucleotides.	MultiMelt